Intranasal administration of cold-adapted live-attenuated SARS-CoV-2 candidate vaccine confers protection against SARS-CoV-2Article Published on 2022-10-022022-11-15 Journal: Virus research [Category] COVID19(2023년), SARS, 진단, [키워드] administration animal model attenuate Attenuated vaccines can be used candidate vaccine cleavage COVID-19 COVID-19 pandemic Deletion elicit examined furin cleavage site furin protease glycoprotein hamster hamsters histopathological Host Immunity Infection lesion motif mucosal neutralizing antibody parental pathogenesis of SARS-CoV-2 polybasic protective immunity proteolytic reduce required robust S1/S2 S1/S2 glycoprotein SARS-CoV-2 SARS-CoV-2. sequence Spike protein subsequent suggested unique Vaccine virus wild-type WT SARS-CoV-2 [DOI] 10.1016/j.virusres.2022.198857 PMC 바로가기
Obatoclax inhibits SARS-CoV-2 entry by altered endosomal acidification and impaired cathepsin and furin activity in vitroCoronaviruses Published on 2022-02-102022-10-31 Journal: Emerging Microbes & Infections [Category] COVID-19, COVID19(2023년), SARS, [키워드] Acidic acute respiratory syndrome Affect antiviral activity Antiviral compound approach B.1.1.7 B.1.351 B.1.617.2 Beta blocked cathepsin L caused Cell clinical virus isolates Compound Concentration coronavirus Coronavirus disease 2019 COVID-19 cysteine protease Delta effective endocytosis Endosomal acidification enzymatic activity expressing furin furin protease global pandemic hACE2 HIV human Angiotensin-converting enzyme human lung in vitro inhibit intestinal cell MCL-1 mechanisms membrane fusion Obatoclax prevented Protein pseudotype reducing reporter S protein Safe SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 spike SARS-CoV-2 variant set off suggested the SARS-CoV-2 Treatment unmet need virion virus virus entry [DOI] 10.1080/22221751.2022.2026739 PMC 바로가기 [Article Type] Coronaviruses
Severity, Pathogenicity and Transmissibility of Delta and Lambda Variants of SARS-CoV-2, Toxicity of Spike Protein and Possibilities for Future Prevention of COVID-19Review Published on 2021-10-182022-10-29 Journal: Microorganisms [Category] COVID-19, [키워드] ACE2 ACE2 receptor amino acid substitution antibody changes in claimed COVID-19 Delta delta variant Effective vaccines Epitopes furin furin protease Future future therapeutic high stability hotspot immune response Lambda Lambda variant less Mutation N protein NAbs neutralizing antibody NTD pathogenicity prevention Protein receptor S protein S proteins SARS-CoV-2 SARS-CoV2 severity spike spike protein toxicity the N protein the S protein Toxicity Treatment TRIM21 vaccinated individuals Vaccine development variant variants virus infection while World Health Organization Wuhan strain [DOI] 10.3390/microorganisms9102167 PMC 바로가기 [Article Type] Review
The structural basis of accelerated host cell entry by SARS-CoV-2†SARS-CoV-2에 의한 가속화된 숙주 세포 진입의 구조적 기초†Article Published on 2021-09-012022-09-11 Journal: The Febs Journal [Category] MERS, SARS, 진단, 치료기술, 치료제, [키워드] accelerate accelerated ACE-2 ACE2-RBD acute respiratory syndrome acute respiratory syndrome coronavirus angiotensin Angiotensin-converting enzyme Antiviral strategies antiviral strategy base C-terminal domain causative agent cell entry cellular entry cleavage site conserved Contact contagious contribute coronavirus Coronavirus disease 2019 COVID-19 disease domain effective entry receptor enzyme epithelial epithelium exhibit explain faster Features flat furin furin protease HCoVs high-affinity binding host cell human coronavirus hypothese Interaction leads Mutation N-terminal domain notable NTD other proteases pandemic pandemic coronavirus Proteases Protein protein-protein interaction RBD receptor Receptor-binding domain respiratory respiratory epithelia S protein S1 subunit SARS-CoV-2 severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 sialic acid sialic acid-binding domain. Spike protein subunit the receptor-binding domain the S protein the S1 subunit the spike protein Tight binding turn Viral viral RBD viral S viral spike [DOI] 10.1111/febs.15651 PMC 바로가기 [Article Type] Article
SARS-CoV-2 Bearing a Mutation at the S1/S2 Cleavage Site Exhibits Attenuated Virulence and Confers Protective ImmunityS1/S2 절단 부위에서 돌연변이를 갖는 SARS-CoV-2는 약화된 독성을 나타내고 보호 면역을 부여합니다Research Article Published on 2021-08-242022-09-10 Journal: mBio [Category] MERS, SARS, 변종, 진단, [키워드] activate affecting airway animal model attenuation B.1.1.7 Bearing body weight Body weight loss cause cell tropism cellular entry cleavage cross-react exhibited furin furin cleavage site furin protease growth hamster hamsters Host immunogens infected with SARS-CoV-2 Infection Inflammatory response inoculated investigated Lineage lineages Mild motif mutant mutants Mutation Neutralizing antibodies P.1 parental Pathogenesis pathogenic pathway polybasic potential benefit potential benefits protective neutralizing antibodies protective neutralizing antibody proteolytic cleavage respiratory S gene S1/S2 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants Severe acute respiratory syndrome severe acute respiratory syndrome-coronavirus-2 Site spike Spike protein subsequent the spike protein tissue variant Viral viral growth weight loss [DOI] 10.1128/mBio.01415-21 PMC 바로가기 [Article Type] Research Article
The COVID‐19 MenaceReview Published on 2021-05-072022-10-31 Journal: Global Challenges [Category] COVID-19, [키워드] acute respiratory syndrome Asymptomatic bind binding site Brain build caused cleavage site Comorbidities complications consequence Consequences contagious coronavirus coronavirus Coronavirus disease 2019 COVID‐19 COVID‐19 determine disease disease spread drugs enzyme furin protease Health immune cells Immunity Infection Local moderate Mortality organs outbreak patients polybasic proteolytic receptors Research SARS‐CoV‐2 SARS‐CoV‐2 sequence severity Spike protein Spread Symptoms the disease tissue tropism transmembrane serine protease Transmission Vaccines Variability virus viruses widespread [DOI] 10.1002/gch2.202100004 PMC 바로가기 [Article Type] Review
Insights on the Structural Variations of the Furin-Like Cleavage Site Found Among the December 2019–July 2020 SARS-CoV-2 Spike Glycoprotein: A Computational Study Linking Viral Evolution and InfectionMedicine Published on 2021-03-102022-10-31 Journal: Frontiers in Medicine [Category] COVID-19, [키워드] absence ACE-2 receptor Amino acid analysis analyzed bind change cleaved Cluster coronavirus disease country COVID-19 deleted Epidemiology Evolution explain furin protease furin-like cleavage site Genome genomic Host host tropism infection clusters insight nine occurred pandemic Protein PRRA RBD SARS-CoV-2 SARS-CoV-2 (SARS2) SARS2 sequence Site spike spike glycoprotein Spike protein Structural variation structural variations the receptor-binding domain unique viral infectivity viral pathogenesis was performed [DOI] 10.3389/fmed.2021.613412 PMC 바로가기 [Article Type] Medicine
Comparison of clinically approved molecules on SARS-CoV-2 drug target proteins: a molecular docking studyArticle Published on 2021-02-172022-10-28 Journal: Turkish Journal of Chemistry [Category] COVID-19, [키워드] affected approved can be used cleave clinically conducted coronavirus COVID-19 docked domain drug design Favipiravir FIVE furin protease globe host cell human ACE2 receptor Hydroxychloroquine inhibitor Interaction molecular molecular docking MOST nucleic acid protease Remdesivir Replication Result Ritonavir SARS-CoV-2 SARS-CoV-2 drug SARS-CoV-2 main protease SARS-CoV-2 spike protein Spike protein target proteins the disease the receptor-binding domain virus Wuhan, China [DOI] 10.3906/kim-2008-35 PMC 바로가기 [Article Type] Article
Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusionSARS-CoV-2 스파이크의 푸린 절단은 감염 및 세포-세포 융합을 촉진하지만 필수적이지는 않습니다Research Article Published on 2021-01-252022-08-13 Journal: PLoS Pathogens [Category] COVID-19, Medicine and Health Sciences, [키워드] ACE2 ACE2 receptor activate activated acute respiratory syndrome coronavirus Amino acid Antiviral antiviral therapies attachment binding Cell cell-cell fusion cells cellular protease cellular proteases cleavage cleaved cocktail coronavirus COVID-19 pandemic CRISPR CRISPR-Cas9 Donor drug focus furin furin cleavage furin inhibitor furin inhibitors furin knockout cell furin knockout cells furin protease fusion fusion peptide host cell host cell membrane host cell receptor infect infected cell Infection inhibitor inhibitors motif multibasic site multinucleated cells mutant Mutation peptide pre-activation Prevent promote protease protease TMPRSS2 Protein reduce reduces required Research respiratory responsible reveal S protein S-mediated cell-cell fusion SARS-CoV-2 SARS-CoV-2 antiviral therapy SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 S SARS-CoV-2 S protein SARS-CoV-2 virus severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus 2 spike Spread syncytia formation target cell the S protein the SARS-CoV-2 thought TMPRSS2 TMPRSS2 protease treat triggered triggering unique Viral viral infection Viral protein viral spread virus virus entry virus entry process virus-host membrane fusion [DOI] 10.1371/journal.ppat.1009246 PMC 바로가기 [Article Type] Research Article
Free SARS-CoV-2 Spike Protein S1 Particles May Play a Role in the Pathogenesis of COVID-19 Infection무료 SARS-CoV-2 스파이크 단백질 S1 입자는 COVID-19 감염의 병인에서 역할을 할 수 있습니다Hypothesis Published on 2020-12-302022-09-01 Journal: Biochemistry. Biokhimiia [Category] SARS, 변종, 진단, [키워드] accompanied ACE ACE2 angiotensin angiotensin (1-7) Angiotensin II Angiotensin-converting enzyme angiotensin-converting enzyme 2 association caused Cell cell surface circulation cleavage site COVID-19 COVID-19 disease COVID-19 infection COVID-19 pathogenesis D614G D614G mutation decrease disease downregulate downregulated Effect expression fraction Free furin furin protease higher infectivity Hypothesis Imbalance in vivo infected cell infected cells Infection Inflammation investigated lack lead less lung Lungs membrane-bound murine model mutant natural infection Particle Pathogenesis pathogenesis of COVID-19 Prevalence Protein pulmonary damage receptor-binding domains Recombinant spike protein Renin renin-angiotensin system reported role S protein S1 and S2 S1 subunit S1 subunit shedding SARS-CoV virus SARS-CoV-2 SARS-CoV-2 S protein SARS-CoV-2 strain separation severity shown spike Spike protein subunits the cell the receptor-binding domain the S protein the S1 subunit the SARS-CoV-2 thrombosis trimer unlikely Viral Viral load virion virions wild type [DOI] 10.1134/S0006297921030032 PMC 바로가기 [Article Type] Hypothesis